Top : Genetics & Genomics : Transgenic Techniques : Embryo Manipulation

Protocols

• Microinjection of Xenopus Oocyte (Gene Delivery Group, University of Birmingham)
  This protocol covers: Preparation of the oocytes, Selection of oocytes for microinjection, Sample preparation, Microinjection of the oocytes, Assaying for gene expression.
  http://web.bham.ac.uk/can4psd4/brum/micro.html
  Added: Mon Sep 09 2002, Hits: 278, Reviews: 0  Write review   Cached
• 1-cell embryo transfer into pseudopregnant recipient female mouse (Gary A. J. Brown)
  Detailed protocol with illustration
  http://ourworld.compuserve.com/homepages/TheBroons...
  Added: Tue May 14 2002, Hits: 245, Reviews: 0  Write review   Cached
• Aggregation of ES Cells and Eight Cell Stage Embryos (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 88, Reviews: 0  Write review   Cached
• Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 167, Reviews: 0  Write review   Cached
• Beta-gal Solution Assay : for quantitative analysis (Mike A. Dyer)
  http://axon.med.harvard.edu/~cepko/protocol/mike/B...
  Added: Tue May 14 2002, Hits: 127, Reviews: 0  Write review   Cached
• Beta-Gal Staining of the Murine Embryo (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
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• Blastocyst Transfer (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
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• Collection of Morulae and Earlier (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 72, Reviews: 0  Write review   Cached
• Embryo Manipulation Pipettes (Flushers etc.) (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 102, Reviews: 0  Write review   Cached
• ES Cell Pipette Manufacture (Gary A. J. Brown)
  Make injection needles yourself
  http://ourworld.compuserve.com/homepages/TheBroons...
  Added: Tue May 14 2002, Hits: 145, Reviews: 0  Write review   Cached
• Flushing Out of Blastocysts (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 81, Reviews: 0  Write review   Cached
• Harvesting of Cells for Blastocyst Injection (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 75, Reviews: 0  Write review   Cached
• Intracytoplasmic Spermatocyte Injection (ICSI) (Brinkmann)
  Detailed technique guide for microinjection.
  http://www.brinkmann.com/ECET_appl4.asp
  Added: Tue May 14 2002, Hits: 64, Reviews: 0  Write review   Cached
• Micropipette Beveling (Gary A. J. Brown)
  http://ourworld.compuserve.com/homepages/TheBroons...
  Added: Tue May 14 2002, Hits: 142, Reviews: 0  Write review   Cached
• PCR Genotyping from the Embryo Yoke Sac (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
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• PCR Genotyping of Embryos and Blastocysts (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 72, Reviews: 0  Write review   Cached
• Preparation of Microinjection Pipettes (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 82, Reviews: 0  Write review   Cached
• Sample Preparation for Microinjection (Brinkmann)
  Microinjection is the loading or transfer of a dissolved substance into a living cell. The microscopic tip of the glass microcapillary has an inner diameter between 0.2 and 1 µm [1]. This capillary is back loaded with the substance to be transferred into the cells cultured for microinjection. Typical substances include purified antibodies, DNA, RNA, peptides, or oligonucleotides.
  http://www.brinkmann.com/ECET_appl2.asp
  Added: Tue May 14 2002, Hits: 79, Reviews: 0  Write review   Cached
• Sending Sera to Testing for Mouse Pathogens (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 41, Reviews: 0  Write review   Cached
• Superovulation of Mice (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 70, Reviews: 0  Write review   Cached
• Vasectomy of Male Mice (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 66, Reviews: 0  Write review   Cached
• Vasectomy of Mouse (Gary A. J. Brown)
  Mice are vasectomized to be mated to females used for embryo transfer. The female becomes pseudopregnant, her body geared for a pregnancy because she has been mated. Vasectomy is performed on mice at 6-8 weeks of age.
  http://ourworld.compuserve.com/homepages/TheBroons...
  Added: Tue May 14 2002, Hits: 141, Reviews: 0  Write review   Cached
• Whole Mount X-gal Histochemistry of Transgenic Animal Tissues and Cultured Cells (Eric Mercer)
  Provides detailed procedures.
  http://www.muridae.com/wmc/docs/lacZ_bible.html
  Added: Tue May 14 2002, Hits: 267, Reviews: 0  Write review   Cached