Protocols
- Microinjection of Xenopus Oocyte (Gene Delivery Group, University of Birmingham)
This protocol covers: Preparation of the oocytes, Selection of oocytes for microinjection, Sample preparation, Microinjection of the oocytes, Assaying for gene expression.
http://web.bham.ac.uk/can4psd4/brum/micro.html
Added: Mon Sep 09 2002, Hits: 970, Reviews: 0 Write review Cached -
1-cell embryo transfer into pseudopregnant recipient female mouse
(Gary A. J. Brown)
Detailed protocol with illustration
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 579, Reviews: 0 Write review Cached -
Aggregation of ES Cells and Eight Cell Stage Embryos
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 222, Reviews: 0 Write review Cached -
Alizarin Red S (Bone) and Alcian Blue (Cartilage) Staining of Cleared Skeletons
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Beta-gal Solution Assay : for quantitative analysis
(Mike A. Dyer)
http://axon.med.harvard.edu/~cepko/protocol/mike/B...
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Beta-Gal Staining of the Murine Embryo
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Blastocyst Transfer
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Collection of Morulae and Earlier
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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Embryo Manipulation Pipettes (Flushers etc.)
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
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ES Cell Pipette Manufacture
(Gary A. J. Brown)
Make injection needles yourself
http://ourworld.compuserve.com/homepages/TheBroons...
Added: Tue May 14 2002, Hits: 240, Reviews: 0 Write review Cached