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Protocols
- Handbook of Immunochemical Staining Methods: : In Situ Hybridization
(Dako)
Introduction / Sample Complexity / Required Sensitivity / Detection Method / Probe Composition / Probe Length / Types of Labels / Labelling Methods
http://pri.dako.com/08002_25may06_ihc_guide_book.p...
Added: Sat Jul 27 2002, Hits: 10069, Reviews: 0
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- In situ Hybridization Protocols (Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
This online book, titled "Practical in situ Hybridization", is aimed at researchers who wish to use efficient and reliable protocols in their work and those who need to check the validity and interpretation of published data. This book presents a reliable, tried-and-tested approach to the methodologies in current use in many laboratories.
http://www.le.ac.uk/biology/phh4/protocols.htm
Added: Fri May 13 2005, Hits: 8713, Reviews: 0
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96-Well RNA In Situ Hybridization
(Berkeley Drosophila Genome Project)
Protocol for fly including embyro preparation, probe labeling, hybridization in 96-well plate.
http://www.fruitfly.org/about/methods/RNAinsitu.ht...
Added: Tue May 14 2002, Hits: 1498, Reviews: 0
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Autoradiography
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
This procedure involves the incorporation of a radioactive substance into a cell, and subsequent detection of that material through the use of a photographic emulsion. The primary source used for cell biology is an organic molecule containing tritium, the radioactive form of hydrogen. Radioactive carbon, phosphorous and iodine are occasionally utilized, but tritium has inherently more resolution than any of the others
http://homepages.gac.edu/~cellab/chpts/chpt2/ex2-8...
Added: Tue May 14 2002, Hits: 898, Reviews: 0
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Chromosome Preparation and Fluorescence in-situ Hybridization (FISH)
(The Science Advisory Board)
http://www.scienceboard.net/resources/protocols.as...
Added: Sun Apr 24 2005, Hits: 2100, Reviews: 0
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Chromosome Preparation and Fluorescence in-situ Hybridization (FISH)
(The Science Advisory Board)
http://www.scienceboard.net/resources/protocols.as...
Added: Sun Jun 05 2005, Hits: 1108, Reviews: 0
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Chromosome Preparation for in situ Hybridization (ISH)
(Wheat Genetics Resource Center)
http://www.ksu.edu/wgrc/Protocols/Cytogenetics/chr...
Added: Tue May 14 2002, Hits: 861, Reviews: 0
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DNA Fiber Spreading for in situ Hybridization
(Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
http://www.le.ac.uk/biology/phh4/dnacomb.htm
Added: Fri May 13 2005, Hits: 428, Reviews: 0
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DNA in situ hybridization with streptavidin-Nanogold and silver acetate autometallography
(Mag. Heidi Bartel)
This protocol allowed a reliable and very sensitive detection of few copies of human papillomavirus (HPV), cytomegalovirus (CMV), Epstein Barr Virus (EBV), herpes simplex virus (HSV) and other DNA-viruses in routinely formalin-fixed and paraffin-embedded tissues.
http://www.sbg.ac.at/kgg/protocols/protocol_1.htm
Added: Tue May 14 2002, Hits: 907, Reviews: 0
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FISH on Human Chromosomes and Interfase Nuclei
(Institute of Pathology,Humboldt-University of Berlin)
Gives details on slide preparation, plasmid, cosmid and YAC library probe preparation and detection
http://users.ugent.be/~fspelema/neubla/protocol/fi...
Added: Tue May 14 2002, Hits: 2276, Reviews: 0
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