Top : Genetics & Genomics : Cytogenetics : In Situ Hybridization

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Protocols

• Handbook of Immunochemical Staining Methods: : In Situ Hybridization (Dako)
  Introduction / Sample Complexity / Required Sensitivity / Detection Method / Probe Composition / Probe Length / Types of Labels / Labelling Methods
  http://www.dakocytomation.us/us_dc_ismhb3_12insitu...
  Added: Sat Jul 27 2002, Hits: 6295, Reviews: 0  Write review  
• 96-Well RNA In Situ Hybridization (Berkeley Drosophila Genome Project)
  Protocol for fly including embyro preparation, probe labeling, hybridization in 96-well plate.
  http://www.fruitfly.org/about/methods/RNAinsitu.ht...
  Added: Tue May 14 2002, Hits: 1370, Reviews: 0  Write review   Cached
• Autoradiography (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
  This procedure involves the incorporation of a radioactive substance into a cell, and subsequent detection of that material through the use of a photographic emulsion. The primary source used for cell biology is an organic molecule containing tritium, the radioactive form of hydrogen. Radioactive carbon, phosphorous and iodine are occasionally utilized, but tritium has inherently more resolution than any of the others
  http://homepages.gac.edu/~cellab/chpts/chpt2/ex2-8...
  Added: Tue May 14 2002, Hits: 1176, Reviews: 0  Write review   Cached
• Chromosome Preparation for in situ Hybridization (ISH) (Wheat Genetics Resource Center)
  http://www.ksu.edu/wgrc/Protocols/Cytogenetics/chr...
  Added: Tue May 14 2002, Hits: 1469, Reviews: 0  Write review   Cached
• DNA in situ hybridization with streptavidin-Nanogold and silver acetate autometallography (Mag. Heidi Bartel)
  This protocol allowed a reliable and very sensitive detection of few copies of human papillomavirus (HPV), cytomegalovirus (CMV), Epstein Barr Virus (EBV), herpes simplex virus (HSV) and other DNA-viruses in routinely formalin-fixed and paraffin-embedded tissues.
  http://www.sbg.ac.at/kgg/protocols/protocol_1.htm
  Added: Tue May 14 2002, Hits: 1202, Reviews: 0  Write review   Cached
• FISH on Human Chromosomes and Interfase Nuclei (Institute of Pathology,Humboldt-University of Berlin)
  Gives details on slide preparation, plasmid, cosmid and YAC library probe preparation and detection
  http://allserv.rug.ac.be/~fspelema/neubla/protocol...
  Added: Tue May 14 2002, Hits: 1904, Reviews: 0  Write review   Cached
• Germline In Situ Hybridization (Schedl Lab)
  In Situ Hybridization to Dissected Gonads with Digoxygenin-Labeled Probes
  http://www.genetics.wustl.edu/tslab/gonad_in_situ....
  Added: Tue May 14 2002, Hits: 744, Reviews: 0  Write review   Cached
• Hiro Hirai's BAC-FISH (Schistosoma Genome Network)
  This protocol is a modification of the standard FISH protocol published by Hiro Hirai and Phil LoVerde that has been optimized for use with BAC probes. This protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labeled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested.
  http://www.nhm.ac.uk/hosted_sites/schisto/protocol...
  Added: Tue May 14 2002, Hits: 430, Reviews: 0  Write review   Cached
• In situ Hybridisation to RNA in Tissue Sections (Bowtell Lab Manual, Peter MacCallum Cancer Institute)
  http://www.pmci.unimelb.edu.au/core_facilities/man...
  Added: Tue May 14 2002, Hits: 917, Reviews: 0  Write review   Cached
• In Situ Hybridisation with DIG-RNA Probes On Embryos (Ilan Davis)
  Protocol for fly including probe preparation
  http://homepages.ed.ac.uk/ilan/methods/insitu.txt
  Added: Tue May 14 2002, Hits: 2070, Reviews: 0  Write review   Cached
• In Situ Hybridization (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
  RNA probe preparation and hybridization for cultured cell
  http://homepages.gac.edu/~cellab/chpts/chpt10/ex10...
  Added: Tue May 14 2002, Hits: 2437, Reviews: 0  Write review   Cached
• In Situ Hybridization (Anderson Lab)
  These protocols describe non-radioactive methods for in situ hybridization on frozen sections, whole mount embryos and on cultured cells.
  http://www.rodentia.com/wmc/docs/Big_In_Situ.html
  Added: Tue May 14 2002, Hits: 3193, Reviews: 0  Write review   Cached
• In Situ Hybridization Method Using DIG Probes (Bissell Lab, Berkely Lab)
  http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
  Added: Sat Feb 21 2009, Hits: 1676, Reviews: 0  Write review   Cached
• In situ hybridization of mammalian cells (Singer Lab)
  in situ hybridization using RNA and Oligonucleotide probes
  http://singerlab.aecom.yu.edu/protocols/insitu_mam...
  Added: Tue May 14 2002, Hits: 2043, Reviews: 0  Write review   Cached
• In Situ Hybridization of Yeast Cells Using RNA and Oligonucleotide Probes (Singer Lab)
  http://singerlab.aecom.yu.edu/protocols/insitu_yea...
  Added: Tue May 14 2002, Hits: 996, Reviews: 0  Write review   Cached
• In Situ Hybridization on Frozen Sections (Wilcox Lab)
  http://www.emory.edu/WILCOX/ishrad.html
  Added: Tue May 14 2002, Hits: 1694, Reviews: 0  Write review   Cached
• In Situ Hybridization to Tissue Sections (Vesicle Trafficking)
  Protocol in great detail
  http://cmgm.stanford.edu/~vesicle/protocols/in_sit...
  Added: Tue May 14 2002, Hits: 2235, Reviews: 0  Write review   Cached
• In Situ Hybridization Using Digoxigenin Labeled Probes (Berkeley Drosophila Genome Project)
  Procedures for chromosome squash preparation, probe labeling, hybridization and more...
  http://www.fruitfly.org/about/methods/cytogenetics...
  Added: Tue May 14 2002, Hits: 1805, Reviews: 0  Write review   Cached
• In Situ  Hybridization (Wheat Genetics Resource Center)
  http://www.ksu.edu/wgrc/Protocols/Cytogenetics/ISH...
  Added: Tue May 14 2002, Hits: 1601, Reviews: 0  Write review   Cached
• In-Situ Hybridization to mRNA using radiolabelled cRNA probes (Beverly Faulkner-Jones)
  In situ hybridization (ISH) is the detection of a target DNA or RNA sequence in a tissue section using a labeled nucleic acid probe. It is still the only hybridization technique which allows cellular and subcellular localization of the target.
  http://wheat.pw.usda.gov/~lazo/methods/au/ish1.htm...
  Added: Tue May 14 2002, Hits: 699, Reviews: 0  Write review   Cached
• Mouse Whole Mount in situ Hybridization (De Robertis Lab, Howard Hughes Medical Institute)
  Protocol including probe labeling (Dig), embryo preparation and hybridization
  http://www.hhmi.ucla.edu/derobertis/protocol_page/...
  Added: Tue May 14 2002, Hits: 413, Reviews: 0  Write review   Cached
• mRNA In Situ Hybridization withNick Translated Probes (Spector Lab)
  http://www.cshl.org/labs/spector/in_situ_hybridiza...
  Added: Tue May 14 2002, Hits: 1046, Reviews: 0  Write review   Cached
• Preparation of Paraffin Sections and Frozen Tissue for FISH (Thomas Ried's Lab, NCI)
  http://www.riedlab.nci.nih.gov/publications/2412.2...
  Added: Fri Oct 18 2002, Hits: 2013, Reviews: 0  Write review  
• Preparation of Stock Solutions for FISH (Institute of Pathology,Humboldt-University of Berlin)
  Recipes for FISH solutions
  http://allserv.rug.ac.be/~fspelema/neubla/protocol...
  Added: Tue May 14 2002, Hits: 1484, Reviews: 0  Write review   Cached
• Protein-DNA Telomere FISH (Stewart Lab, Washington University at St. Louis)
  http://www.stewartlab.net/Protocols/Telomere_Fish_...
  Added: Thu May 27 2004, Hits: 1055, Reviews: 0  Write review   Cached

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