Protocols
- Bradford Assay (Kitto Lab, The University of Texas at Austin)
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein.
http://kitto.cm.utexas.edu/research/Kittolabpage/P...
Added: Tue Oct 08 2002, Hits: 30363, Reviews: 0 Write review Cached - Bradford Protein Concentration Assay (Dr. Mario Lebendiker, The Hebrew University of Jerusalem)
A rapid and accurate method for the estimation of protein concentration. The technique is simpler, faster than the Lowry method, and is subject to less interference.
http://wolfson.huji.ac.il/purification/Protocols/B...
Added: Tue Feb 17 2009, Hits: 22255, Reviews: 0 Write review Cached - Bradford Protein Concentration Assay (Dr. Loren Williams, Georgia Institute of Technology)
Step-by-step protocol and recipe for standards.
http://web.chemistry.gatech.edu/~williams/bCourse_...
Added: Tue Feb 17 2009, Hits: 16348, Reviews: 0 Write review Cached - Bradford Protein Concentration Assay (School of Chem. and Biochem, Georgia Institutue of Technology)
The Bradford protein assay is one of several simple methods commonly used to determine the total protein concentration of a sample. The method is based on the proportional binding of the dye Coomassie to proteins. Within the linear range of the assay (~5-25 mcg/mL), the more protein present, the more Coomassie binds...
http://ww2.chemistry.gatech.edu/~lw26/bCourse_Info...
Added: Sat Aug 04 2012, Hits: 4907, Reviews: 0 Write review -
BioRad Protein Assay: Bradford Reagent
(Suprya Jayadev)
http://www.musc.edu/bcmb/ceramide/protocols/0019.h...
Added: Tue May 14 2002, Hits: 8617, Reviews: 0 Write review Cached -
Bradford Assay
(South African Structural Biology Network)
Brief introduction, references, reagents and step-by-step protocol.
http://sbio.uct.ac.za/Sbio/documentation/bradford_...
Added: Tue Feb 17 2009, Hits: 2832, Reviews: 0 Write review Cached -
Bradford Protein Assay
(P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
The Bradford protein assay is a simple procedure for determination of protein concentrations in solutions that depends upon the change in absorbance in Coomassie Blue G-250 upon binding of protein. Unlike many other assays, including the Lowry procedure, the Bradford assay is not susceptible to interference by a wide variety of chemicals present in samples. The notable exception is high concentrations of detergents. There is significant protein-to-protein variation in absorbance values obtained with the Bradford procedure and it is advisable to choose a protein standard that is likely to give absorbance values close to those for the protein samples of interest. The assay here is designed for use in microtiter plates. This is an easy assay format for those with access to multiple channel pipettors and microtiter plate spectrophotometers.
http://www.animal.ufl.edu/hansen/protocols/minibra...
Added: Tue May 14 2002, Hits: 13373, Reviews: 0 Write review Cached -
Bradford Protein Assay
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/appds/appd-g.html
Added: Tue May 14 2002, Hits: 6884, Reviews: 0 Write review Cached -
Bradford Protein Assay
(David R. Caprette, Rice University)
Detailed procedure with background info and comments.
http://www.ruf.rice.edu/~bioslabs/methods/protein/...
Added: Tue Feb 17 2009, Hits: 7039, Reviews: 0 Write review Cached