Protocols
- Plate Assay for Determination of DNA Concentration (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
A fairly accurate, rapid assay of DNA concentration can be obtained by UV visualization of samples spotted onto ethidium bromide-containing agarose plates.
http://www.nottingham.ac.uk/~mbzspd/methods/Plate_...
Added: Sat Apr 10 2004, Hits: 280, Reviews: 0 Write review Cached - Quantification and Quality
(CIMMYT Applied Molecular Genetics Laboratory)
Nice protocol on DNA UV quantification, quality control, digestibility test.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 8439, Reviews: 0 Write review -
Characterization of DNA
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
DNA quantitation by spectrophotometer
http://homepages.gac.edu/~cellab/chpts/chpt14/ex14...
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Determination of DNA Concentration
(Gene Delivery Group, University of Birmingham)
Determination of DNA concentration using the spectrophotometer
http://web.bham.ac.uk/can4psd4/dnaconc.html
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DNA concentration assay with fluorescent dye
(The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/dn...
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DNA Quantification by Spot Fluorescence
(CIMMYT Applied Molecular Genetics Laboratory)
Using UV Transilluminator and EtBr
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Mon Aug 05 2002, Hits: 1815, Reviews: 0 Write review -
DNA Quantification by TCA Precipitation
(John Roth, Division of Biological Sciences,University of California at Davis)
http://rothlab.ucdavis.edu/protocols/Z01.html
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DNA Quantification Using the TKO 100 MiniFluorometer
(CIMMYT Applied Molecular Genetics Laboratory)
Protocol in nice format.
http://www.cimmyt.org/english/docs/manual/protocol...
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DNA Quantitation
(Gerard Lazo)
Including spot test and DPN assay
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaqua...
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Estimation of DNA Concentration
(LaboratoryExperiments.com)
A basic procedure similar to spot test for estimating DNA concentration using SYBR Green I(R) nucleic acid gel stain in stead of EB.
http://www.laboratoryexperiments.com/LAB4.htm
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Hoechst Fluorometric Quantitation of DNA
(Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
The concentration of DNA in solutions containing contaminating protein or RNA can be determined quite accurately by measuring the increased fluorescence of the Hoechst dye 33258 upon intercalating into dsDNA.
http://www.nottingham.ac.uk/~mbzspd/methods/Hoechs...
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Quantification of Nuclear Acid and Protein
(Brinkmann)
Some basics about nuclear acid and protein quantification by spectrophotometer.
http://www.brinkmann.com/quant_easy.asp
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Quantitation of DNA for Sequencing Using Fluorometer
(Hancock Lab)
(Accessible only by IE)
http://www.cmdr.ubc.ca/bobh/methods/QUANTITATIONOF...
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Quantitation of Nucleic Acid Concentration
(Faulkner-Jones)
Three methods are provided for nucleic acid quantitation: optical density measurements; ethidium bromide - agarose plates, and agarose gel electrophoresis estimation with a known standard DNA.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
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Spot Test
(Schneitz Lab)
This technique provides a rapid way to make a rough but useful estimate of the DNA concentration of a given sample (e.g. isolated fragment).
http://plantdev.bio.wzw.tum.de/index.php?id=77
Added: Tue May 14 2002, Hits: 1372, Reviews: 0 Write review Cached