1) Samples to be measured should be diluted to 1ml in H2O or TE, pH8.0.
2) 1ml of Hoechst buffer (4M NaCl, 100mM NaP04, pH7.4, 0.2ug/ml Hoechst 33258) is added to each sample.
3) Measure fluorescence at an emission wavelength of 458nM and an excitation wavelength of 356nM.
DNA concentrations can be measured by comparison of the emission intensities of the unknown samples with those generated by a series of samples of known DNA concentrations.
Dr. Simon Dawson,
Department of Biochemistry,
University of Nottingham,
The Medical School ,
Clifton Boulevard ,
Tel: +44 (0)115 9249924 ex.44787,
FAX: +44 (0)115 9422225,
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