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Protocols
- Molecular Weight Markers for Gel Electrophoresis
(CIMMYT Applied Molecular Genetics Laboratory)
Two types of molecular weight (MW) standards are routinely used. The Lambda/HindIII and
PhiX174/HaeIII MW standards provide a useful reference for calculating molecular weights of large and small DNA fragments, respectively, after electrophoretic separation; the ́internal MW standards provide a means for normalizing fragment migration distances within each lane to facilitate comparisons between lanes on the same or different luminographs in fingerprinting studies.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 5850, Reviews: 0
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Molecular Weight Marker
(lazo Lab)
For making Hind III digestion marker
http://wheat.pw.usda.gov/~lazo/methods/lazo/molwts...
Added: Tue May 14 2002, Hits: 3383, Reviews: 0
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Molecular Weight Markers
(BioProtocol)
List of commonly used molecular weight markers
http://www.bioprotocol.com/protocolstools/molecula...
Added: Sun Jul 21 2002, Hits: 4839, Reviews: 0
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Preparation of DNA ladder for agarose gels
(IzziD wetlab)
When you first start in a lab, it can be tricky to learn how to appropriately dilute the concentrated DNA ladders provided by experimental biology suppliers into a form suitable for agarose gel electrophoresis. You must dilute the DNA ladder stock with the appropriate amounts of TE and agarose gel loading dye. If you don't want to spend time preparing your DNA ladder, many biosupply companies provide ready-to-load ladders for about 2x the cost of concentrated ladder.
http://wetlab.izzid.com/2007/Oct/Make_DNA_Ladder/
Added: Sat Oct 27 2007, Hits: 731, Reviews: 0
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