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Molecular Weight Marker

Molecular Weight Marker

l HindIII Digest Marker Solution

  1. Digest 20 µg l DNA (40 µl DNA if at 0.5 µg/µl)
  2. Add 50 µl 10X Tracking Dye
  3. Bring volume of the digested DNA up to 500 µl with H2O (410 µl if the DNA is 40 µl): If l DNA is digested in our lab, the volume of H2O varys.
  4. Mix, heat to 65 C for 5 min, cool quickly on ice, and store at 4 C.
  5. Load 5 µl (200 ng per application)

l Digest Marker DNA Size (in base pair)s and Quantity (5 µl application per lane)

 PstI	BstII	HindIII  11,497	8,454	23,130	95 ng DNA   5,077	7,242	 9,416	38.8   4,749	6,369	 6,557	27   4,507	5,686	 4,507	18.5   2,828	4,822	 2,322	 9.6   2,559	4,324	 2,027	 8.4   2,443	2,323	   125   2,140	1,929   1,986	1,371   1,700	1,264   1,159	  702   1,093	  224     795	  224     514      468     448     339     274     247     216     211     200     164     150      94      87      75      72      
Molecular Weight Hybridization Marker