Protocols
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hCG Injection
(Harland Lab, UC Berkely)
Injecting trops with hCG to induce ovulation
http://tropicalis.berkeley.edu/home/obtaining_embr...
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In Vitro Fertilization of X. tropicalis
(Harland Lab, UC Berkely)
http://tropicalis.berkeley.edu/home/obtaining_embr...
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Isolating Frog Testis
(Harland Lab, UC Berkely)
Isolating testis for in vitro fertilization (IVF) of eggs
http://tropicalis.berkeley.edu/home/obtaining_embr...
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Natural Matings
(Harland Lab, UC Berkely)
how to setup matings between males and femals to harvest eggs
http://tropicalis.berkeley.edu/home/obtaining_embr...
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Sea Urchin Fertilization
(Experiments in Developmental Biology)
http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/U...
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Sea Urchin Gamete collection
(Experiments in Developmental Biology)
http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/U...
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Squeezing Females for Eggs
(Harland Lab, UC Berkely)
Squeezing females to get eggs for IVF
http://tropicalis.berkeley.edu/home/obtaining_embr...
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X. tropicalis Mating Protocol
(Grainger Lab, University of Virginia)
This protocol is designed to produce the maximum number of offspring from a natural male/female mating. It uses artificial stimulation by HCG to induce maturation of gametes and mating behaviors.
http://www.faculty.virginia.edu/xtropicalis/husban...
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Xenopus Tropicalis Sperm Freezing
(Harland Lab, UC Berkely)
X. tropicalis sperm can be frozen for long term storage of lines and then later thawed to recover the line.
http://tropicalis.berkeley.edu/home/obtaining_embr...
Added: Wed Mar 04 2009, Hits: 1266, Reviews: 0 Write review Cached