Subcategories

Protocols

• Cell number counting in preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
  A simple procedure for determining cell number in preimplantation embryos. While the procedure is written for fixed embryos, fixation is optional since the Hoechst 33342 and 4',6-diamidino-2-phenylindole, dihydrochloride (DAPI) penetrate living cells. The fixation step is useful when embryos will be stored for several weeks before determination of cell number
  http://www.animal.ufl.edu/hansen/protocols/embryoc...
  Added: Tue May 14 2002, Hits: 1444, Reviews: 0  Write review   Cached
• Detection of Reactive Oxygen Species in Preimplantation Bovine Embryos (E. Pontes and P.J. Hansen, University of Florida, PJ Hansen laboratory)
  A procedure for measuring free radical production in embryos based on the oxidation of 5-(and-6)-carboxy-2',7'-dihydrofluorescein diacetate is described. The procedure is written for use with embryos but can be easily modified for use with other cells.
  http://www.animal.ufl.edu/hansen/Protocols/Detecti...
  Added: Fri Oct 26 2007, Hits: 251, Reviews: 0  Write review  
• Differential staining of trophectoderm and inner cell mass in combination with TUNEL labeling of bov (P.J. Hansen Laboratory, University of Florida)
  A procedure for determining TUNEL positive cells in ICM and TE cells of blastocysts
  http://www.animal.ufl.edu/hansen/Protocols/Triples...
  Added: Thu Mar 24 2005, Hits: 616, Reviews: 0  Write review   Cached
• In Vitro Production of Bovine Embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
  This protocol describe procedures for in vitro production [IVP; i.e. in vitro maturation (IVM), in vitro fertilization (IVF), and in vitro culture (IVC)]of embryos
  http://www.animal.ufl.edu/hansen/IVF/default.htm
  Added: Tue May 14 2002, Hits: 863, Reviews: 0  Write review   Cached
• Lineage Analysis Using Retrovirus Vectors (Cepko Lab, Department of Genetics, Harvard Medical School and Howard Hughes Medical Institute,)
  Describe the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues.
  http://axon.med.harvard.edu/~cepko/lineage/
  Added: Tue May 14 2002, Hits: 3288, Reviews: 0  Write review   Cached
• Mini-Chamber for regulating gaseous environment during culture (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
  A simple and inexpensive chamber for regulating gaseous environment of small culture plates, such as those used for culture of preimplantation embryos, can be constructed using disposable media-filtration devises such as Corning’s 115-ml system. The following is a description of how to make such a device
  http://www.animal.ufl.edu/hansen/protocols/minigas...
  Added: Tue May 14 2002, Hits: 485, Reviews: 0  Write review   Cached
• TUNEL procedure for preimplantation embryos (P.J. Hansen Lab, Dept. of Animal Sciences, University of Florida)
  A protocol for detecting cell death using TUNEL.
  http://www.animal.ufl.edu/hansen/protocols/tunel.h...
  Added: Tue May 14 2002, Hits: 1155, Reviews: 0  Write review   Cached