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Pheromone Halo Assay
(Henrik G. Dohlman, The University of North Carolina Chapel Hill)
This is a bio-assay that measures the responsiveness of cells to a factor pheromone. The assay is easy to conduct and the results are usually unambiguous and highly reproducible.
http://www.med.unc.edu/%7Ehdohlman/haloassay.html
Added: Sun Aug 18 2002, Hits: 735, Reviews: 0
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SAPK/Jun kinase assays
(Woodgett Lab,Ontario Cancer Institute/Princess Margaret Hospital)
Detailed protocol including cell lysate preparation, assay and receipes.
http://kinase.uhnres.utoronto.ca/methods/SAPKassay...
Added: Thu Aug 01 2002, Hits: 1977, Reviews: 0
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T-Cell Activation Using mAb to CD3
(eBioscience)
This protocol is written as a starting point for examining in vitro proliferation of splenic T-cells stimulated via CD3. All the steps where optimization by the user can be achieved for meaningful results have been highlighted. Briefly, these include titration of responding cells, titration of the activating reagents and the optimal culture period for the best activation response. MTT assay is used for the detection of cellular proliferation.
http://www.ebioscience.com/ebioscience/appls/AC145...
Added: Tue May 14 2002, Hits: 2826, Reviews: 0
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