Isolation of Crude Nuclear Pellets
(Fungal Genetics Stock Center)
This procedure describes a convenient method for the isolation of crude nuclear pellets from N. crassa. The method utilizes a Braun homogenizer to disrupt the cells. The main advantages of the technique are that the cells need not be froen, large amounts of material can be handled, the homogenization is fast and easily controlled, fewer omni-mix steps and shoter times are required to release the nuclei, yields are comparable (75%) to those obtained using the french pressure cell, and lower concentrations of Ficoll will stabilize the nuclei.
Added: Mon Dec 09 2002, Hits: 950, Reviews: 0