Protocols
- Isolation of Interphase Nuclei (Thomas Ried's Lab, NCI)
Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique.
http://www.riedlab.nci.nih.gov/publications/2408.2...
Added: Fri Oct 18 2002, Hits: 591, Reviews: 0 Write review -
Adrenal Chromaffin Granule (chromaffin vesicle) Preparation
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
This method is usually used to prepare granules from freshly obtained (never frozen!) bovine adrenal glands, but can also be used to prepare chromaffin granules from fresh human pheochromocytoma.
http://hypertension.ucsd.edu/list_of_protocols/17_...
Added: Tue May 14 2002, Hits: 161, Reviews: 0 Write review Cached -
Cell Disruption and Subcellular Fractionation
(Hannun/Obeid Lab)
http://www.musc.edu/bcmb/ceramide/protocols/0011.h...
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Centrosome Preparation
(Mitchison Lab)
The protocol is identical to Tim's published in Vol 134 of Methods in Enzymology. The key step is the lysis which solubilizes centrosomes away from nuclei by very low ionic strength lysis after treatment of cells with nocodazole and cytochalasin B. The released centrosomes are then centrifuged onto a Ficoll cushion (to avoid pelleting) and the interface between the lysate and the Ficoll is collected and the centrosomes are concentrated on a sucrose gradient.
http://mitchison.med.harvard.edu/protocols/mt1.htm...
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Crude Extract Preparation for In Vitro Spindle Assembly
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/ext3.ht...
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Golgi vesicle preparation from pea etiolated hypocotyls
(Ivan J Delgado)
http://ivaan.com/protocols/171.html
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Large Scale Tubulin Preparation
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/tubprep...
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Lysosome Isolation in Isotonic Sucrose
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt7/ex7-1...
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Preparation of Rat Liver Cell Cytosol and/or
(Salmon Lab )
Organelle Fractions (Salmon Lab)
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
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Preparation of tubulin
(Salmon Lab)
Simple procedure with high yielding
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
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Tubulin Basics
(Mitchison Lab)
Values about tubulin and how to handle tubulin
http://mitchison.med.harvard.edu/protocols/basics....
Added: Tue May 14 2002, Hits: 204, Reviews: 0 Write review Cached