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Protocols
- Cajal Body Isolation (Lamond Lab, University of Dundee )
The Cajal body (CB) is a conserved, dynamic nuclear structure that is implicated in various cellular processes, such as the maturation of splicing small nuclear ribonucleoproteins and the assembly of transcription complexes. Here is the procedure for the large-scale purification of CBs from HeLa cell nuclei, resulting in an ~750-fold enrichment of the CB marker protein p80-coilin. Also available are Quicktime movies demonstrating key steps of the protocol
http://www.lamondlab.com/f5cbprotocol.htm
Added: Thu Nov 14 2002, Hits: 404, Reviews: 0 Write review Cached -
Adrenal Chromaffin Granule (chromaffin vesicle) Preparation
(Daniel T. O'Connor, UCSD Chromaffin Cell and Hypertension Research )
This method is usually used to prepare granules from freshly obtained (never frozen!) bovine adrenal glands, but can also be used to prepare chromaffin granules from fresh human pheochromocytoma.
http://medicine.ucsd.edu/hypertension/list_of_prot...
Added: Tue May 14 2002, Hits: 369, Reviews: 0 Write review Cached -
Cell Disruption and Subcellular Fractionation
(Hannun/Obeid Lab, Duke Univ.)
http://www.duke.edu/web/ceramide/protocols/0011.ht...
Added: Sun Apr 24 2005, Hits: 2268, Reviews: 0 Write review Cached -
Em Observations of Microsomes
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt7/ex7-2...
Added: Tue May 14 2002, Hits: 513, Reviews: 0 Write review Cached -
Lysosome Isolation in Isotonic Sucrose
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt7/ex7-1...
Added: Tue May 14 2002, Hits: 743, Reviews: 0 Write review Cached -
Preparation of Rat Liver Cell Cytosol
(Salmon Lab, Department of Biology, University of North Carolina at Chapel Hill)
Organelle Fractions (Salmon Lab)
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
Added: Tue May 14 2002, Hits: 2313, Reviews: 0 Write review Cached -
Preparation of tubulin
(Salmon Lab, Department of Biology, University of North Carolina at Chapel Hill)
Simple procedure with high yielding
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
Added: Tue May 14 2002, Hits: 508, Reviews: 0 Write review Cached