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Protocols
- Cell Cycle and Apoptosis Analysis (Miriam Capri and Daniela Barbieri)
This protocol describes four different flow cytometric methods, two for the discrimination of cell cycle phases and two for the simultaneous assessment of cell cycle and apoptosis.
http://www.immunologia.unimore.it/SITO%20AC/APOBOO...
Added: Thu Mar 08 2007, Hits: 3376, Reviews: 0
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Cell Cycle
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Introduction to cell cycle knowledge
http://homepages.gac.edu/~cellab/chpts/chpt11/intr...
Added: Tue May 14 2002, Hits: 9020, Reviews: 0
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Cell Cycle Analysis
(Flow Cytometry Core Facility, PrincetonUniversity)
Three methods are described including PROPIDIUM IODIDE, ACRIDINE ORANGE and HOECHST/DAPI methods
http://www.molbio.princeton.edu/facility/flowcyt/C...
Added: Tue May 14 2002, Hits: 7070, Reviews: 0
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DNA Staining for Cell Cycle Analysis
(The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
Propidium iodide (PI) staining of DNA in cultured cells or cells from tissue
http://cyto.mednet.ucla.edu/Protocols/dnapi.htm
Added: Sat Feb 11 2006, Hits: 4971, Reviews: 0
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H-Thymidine Uptake by Cultured Cells
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
Added: Tue May 14 2002, Hits: 1637, Reviews: 0
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Preparing Cells for PI/FACS (Cell Cycle) Analysis
(The Flemington Lab, Tulane Health Sciences Center)
This method can be used to assess cell cycle distribution of whole cell populations and can also be used to assess the cell cycle distribution of GFP transfected cells
http://www.flemingtonlab.com/Protocols/PreparingCe...
Added: Thu Jun 16 2005, Hits: 5453, Reviews: 0
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Sub-G1 Propidium Iodide Staining
( FACS Laboratory, London Research Institute)
http://science.cancerresearchuk.org/sci/facs/facs_...
Added: Fri Jun 17 2005, Hits: 3320, Reviews: 0
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Timing of Cycles
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
Determine Cell cycle by exposing cell to 3H-Thymidine, and subsequently fixing and autoradiography.
http://homepages.gac.edu/~cellab/chpts/chpt11/ex11...
Added: Tue May 14 2002, Hits: 3118, Reviews: 0
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