Protocols

• BrDU and PI Staining for Cell Cycle Analysis (Fero Lab, Fred Hutchinson Cancer Research Center)
  http://www.fhcrc.org/science/labs/fero/Protocols/B...
  Added: Fri Jul 20 2007, Hits: 1339, Reviews: 0  Write review   Cached
• Cell Cycle Analysis by Propidium Iodide (PI) Staining (Johannes A. Schmid, Medical University Vienna)
  Describes different methods for adherent cells and suspension cells and methods for fixation.
  http://www.univie.ac.at/VascBio/schmid/PIstaining3...
  Added: Fri Jul 20 2007, Hits: 2486, Reviews: 0  Write review   Cached
• Cell Cycle Analysis of DNA Content by Propidium Iodide (PI) Staining (UCSD Cancer Center Flow Cytometry)
  Cells are fixed and stained with PI for cell cycle analysis
  http://cancer.ucsd.edu/flow/Protocols/DNA%20Conten...
  Added: Thu Jul 10 2008, Hits: 138, Reviews: 0  Write review  
• Ethanol-fixation of Samples for Long-term Storage and Subsequent DNA Staining (The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
  Procedure for cell fixation and staining.
  http://cyto.mednet.ucla.edu/Protocols/ethanol.htm
  Added: Sat Feb 11 2006, Hits: 3254, Reviews: 0  Write review  
• Fixation and DNA Staining for Cell Cycle Analysis (Iowa State University, Flow Cytometry Facility)
  This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells.
  http://www.biotech.iastate.edu/facilities/CELLHYB/...
  Added: Tue May 14 2002, Hits: 7722, Reviews: 0  Write review   Cached
• Flow Cytometric Analysis of Cell Cycle (Hannun/Obeid Lab)
  Protocol for fixing and staining
  http://www.musc.edu/bcmb/ceramide/protocols/0010.h...
  Added: Tue May 14 2002, Hits: 5568, Reviews: 0  Write review   Cached
• Preparing Cells for PI / FACS (cell cycle) Analysis (The Flemington Lab, Tulane Health Sciences Center)
  http://www.flemingtonlab.com/Protocols/PreparingCe...
  Added: Fri Jul 20 2007, Hits: 1608, Reviews: 0  Write review  
• Propidium Iodide (PI) Staining of Dead Cells (The Janis V. Giorgi Flow Cytometry Laboratory, UCLA)
  Propidium iodide (PI) intercalates into double-stranded nucleic acids. It is excluded by viable cells but can penetrate cell membranes of dying or dead cells.
  http://cyto.mednet.ucla.edu/Protocols/pi.htm
  Added: Sat Feb 11 2006, Hits: 3200, Reviews: 0  Write review   Cached
• Propidium Iodide (PI) Staining Protocols (AMS Flow Cytometry Core Facility)
  Two protocols are described.
  http://www.uams.edu/flowcytometry/folderone/PI.asp
  Added: Fri Jul 20 2007, Hits: 2231, Reviews: 0  Write review