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Protocols
- Cell Cycle Analysis by DNA Content (Propidium Iodide) (UCSD Cancer Center Flow Cytometry Core Facility)
Classic cell cycle analysis by PI staining with example graphics
http://cancer.ucsd.edu/flow/Protocols/DNA%20Conten...
Added: Sun Aug 01 2010, Hits: 4086, Reviews: 0 Write review Cached -
DNA Content Detection for Cell Cycle Analysis
(Cell Sorter Facility, Yale University)
http://info.med.yale.edu/immuno/cytometry/docs/DNA...
Added: Mon Feb 23 2009, Hits: 4214, Reviews: 0 Write review Cached -
FACS Cell Cycle Staining using DAPI
(Flow Cytometry Core Facility, University of Chicago)
http://iacf.uchicago.edu/Protocols/DAPICellCyclePr...
Added: Sat Feb 21 2009, Hits: 2754, Reviews: 0 Write review Cached -
Fixation and DNA Staining for Cell Cycle Analysis
(U of Florida Flow Cytometry Core Laboratory)
This method of DNA staining utilizes ethanol to fix the cells and permeabilize the membrane, which allows the dye (Propidium Iodide) to enter the cells.
http://www.biotech.ufl.edu/FlowCytometry/dna_fix.h...
Added: Tue May 14 2002, Hits: 1229, Reviews: 0 Write review Cached -
Flow Cytometric Analysis of Cell Cycle
(Hannun/Obeid Lab)
Protocol for fixing and staining
http://www.musc.edu/bcmb/ceramide/protocols/0010.h...
Added: Tue May 14 2002, Hits: 4375, Reviews: 0 Write review Cached -
Flow Cytometry of Fibroblast Nuclei for DNA content
(Fero Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/fero/Protocols/MEFnuclei.htm...
Added: Sat Jan 31 2009, Hits: 1139, Reviews: 0 Write review Cached -
PI Labeling Method for FACS Analysis
(Chen Lab, UCSF)
http://bts.ucsf.edu/chen/Protocol/PI%20Labeling%20...
Added: Sun Oct 25 2009, Hits: 4053, Reviews: 0 Write review -
Propidium Iodide Staining for DNA Ploidy
(Bissell Lab, Berkely Lab)
http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
Added: Sat Feb 21 2009, Hits: 2894, Reviews: 0 Write review -
Propidium Iodide Staining of Dead Cells For FACS Analysis
(UCLA Flow Cytometry Core)
http://cyto.mednet.ucla.edu/Protocols/pi.htm
Added: Sat Jan 31 2009, Hits: 5163, Reviews: 0 Write review Cached