Protocols

• Medium Change for Suspension Cell Culture (ATCC)
  http://www.atcc.org/TechnicalInfo/faqCellBiology.c...
  Added: Thu Mar 22 2007, Hits: 3698, Reviews: 0  Write review   Cached
• Subculture of Suspension Cell Lines (Sigma)
  In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of lines subculture by dilution is relatively easy. But for lines that grow in clumps it may be necessary to bring the cells into a single cell suspension by centrifugation and resuspension by pipetting in a smaller volume before counting.
  http://www.sigmaaldrich.com/Area_of_Interest/Life_...
  Added: Sun Jul 04 2004, Hits: 8007, Reviews: 0  Write review   Cached

Trouble Shootings and FAQs

• How to seperate dead cells from living ones in suspension culture?
• How to seperate dead suspension cells from alive one
• Can suspension culture be transformed into adherent cells?
• How to handle suspension cells?
• How to attach suspension cell to coverslips?
• Suspension Cell Culture - How to removal of dead cells?
• MTT test for the suspension culture - Raji cell and K562
• Transfection of suspension cells - how to improve the transfection efficiency?