Protocols
- Subculture of Suspension Cell Lines (Sigma)
Cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed lymphoblastoid cell lines). For these types of lines subculture by dilution is relatively easy. But for lines that grow in clumps it may be necessary to bring the cells into a single cell suspension...
http://www.sigmaaldrich.com/life-science/cell-cult...
Added: Fri Feb 06 2009, Hits: 9333, Reviews: 0 Write review Cached -
Growth, Maintenance and Transfection of Suspension Adapted Cells
(The Science Advisory Board)
This protocol describes the instructions for the growth, maintenance and transfection of suspension adapted 293-EBNA cell line using the PEI expression system.
http://www.scienceboard.net/resources/protocols.as...
Added: Thu Feb 19 2009, Hits: 4092, Reviews: 0 Write review Cached -
Transfer of Eukaryote Suspension Cultures
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt12/ex12...
Added: Tue May 14 2002, Hits: 2335, Reviews: 0 Write review Cached