A tissue culture hood is a device that has air moving in layers and under positive pressure. Since the air is filtered, it contains minimal numbers of bacteria or fungal spores, and since it is under a positive pressure, those particles that are present are blown out of the hood. The layering prevents airborne organisms from settling on the work surfaces.
Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask.
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