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Protocols
- Establishing Cell Line from Tumors (Cowin Lab, New York University Medical Center)
Method for creating cell lines from tumor tissue
http://celljunctions.med.nyu.edu/lab/notprivate/me...
Added: Mon Mar 16 2009, Hits: 3673, Reviews: 0 Write review Cached - Establishment of a Primary Culture (Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt12/ex12...
Added: Tue May 14 2002, Hits: 8508, Reviews: 0 Write review Cached - Primary Mammary Epithelial Isolation (Sissell Lab, Lawrence Berkeley National Laboratory)
Primary mammary epithelial cell isolation from mouse mammary gland.
http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
Added: Sat Apr 10 2010, Hits: 1422, Reviews: 0 Write review Cached -
Culture of Endometrial Explants
(P.J. Hansen Lab)
This method is used to study secretion of proteins and prostaglandins by endometrium from the cow, ewe, mare, bitch and other species. The technique is also useful for culture of peri-implantation conceptuses and placental tissues for metabolic labelling studies and to obtain conceptus secretory proteins for biological studies.
http://www.animal.ufl.edu/hansen/protocols/endocul...
Added: Tue May 14 2002, Hits: 858, Reviews: 0 Write review Cached -
Direct tissue Explant for Derivation and Study of Extravillous Trophoblast
(Aplin Laboratory, Reproductive Biology at the University of Manchester )
This method enables the researcher to explant terminal placental villi onto a three dimensional matrix of either collagen or Matrigel and monitor the formation of anchoring cytotrophoblast columns. The resulting cells can be stimulated to assume a migratory phenotype by growth factors. TGFb1 inhibits outgrowth.
http://www.medicine.man.ac.uk/aplin/protocols1.htm...
Added: Fri Dec 13 2002, Hits: 656, Reviews: 0 Write review Cached - Freezing and Thawing of MEFs
This protocol is very useful to the researchers working on primary mouse embryonic fibrobasts and gene cell lines
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Slice and Explant Culture
(Robert F. Hevner Lab, University of Washington School of Medicine)
Detailed method with media and solutions
http://faculty.washington.edu/rhevner/Slice_Cultur...
Added: Fri Dec 13 2002, Hits: 1701, Reviews: 0 Write review -
Staining Protocols for Explant Cultures
(Aplin Laboratory, Reproductive Biology at the University of Manchester )
The protocol uses a single primary antibody with nuclear counterstaining, but it can be extended to double antibody staining. It requires an inverted microscope with fluorescence illumination and long working distance objectives.
http://www.medicine.man.ac.uk/aplin/protocols2.htm...
Added: Fri Dec 13 2002, Hits: 1530, Reviews: 0 Write review Cached