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Protocols
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Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
(JoVE)
This video protocol demonstrates a micro-dissected explants technique, a robust and reliable method for isolating relatively large numbers of proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells.
http://www.jove.com/details.php?id=2051
Added: Mon May 16 2011, Hits: 810, Reviews: 0 Write review -
Blocking Endogenous Biotin
(The Molecular Probes Technology Network)
Endogenous Biotin-Blocking
http://molecularprobestechnologynetwork.community....
Added: Fri Oct 15 2010, Hits: 706, Reviews: 0 Write review -
Enrichment of PBMCs with monocytes
(The Science Advisory Board)
This protocol is used in our lab to reduce the costs of the cell sorting with MACS reagents. The cell suspension obtained after this protocol contains 40-70% monocytes. This cell suspension is than used for positive or negative MACS separation.
http://www.scienceboard.net/resources/protocols.as...
Added: Thu Feb 19 2009, Hits: 1725, Reviews: 0 Write review Cached -
Harvesting peritoneal cells
(Giorgio Cattoretti, MD, Columbia University )
Surgical procedure for collecting mouse peritoneal cells
http://icg.cpmc.columbia.edu/cattoretti/Protocol/M...
Added: Sat Jul 27 2002, Hits: 1321, Reviews: 0 Write review Cached -
Human Peripheral Blood Mononuclear Cell Preparation
(Bio.com)
This protocol describes a procedure for isolating human peripheral blood mononuclear cells (lymphocytes and monocytes) from a Buffy Coat (obtained from a blood bank).
http://bioprotocol.bio.com/protocolstools/protocol...
Added: Fri Oct 17 2003, Hits: 750, Reviews: 0 Write review Cached - In-Vitro Adipocytes Differentiation
This protocol is very useful for researchers working in the field of obesity, diabetes, CVD especially in adipocyte biology. Here it has been descirbed with its importance and basic principle of the assay
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Isolating Xenograft Tumor Cells for Tissue Culture or Transplantation
(Bissell Lab, Berkely Lab)
http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
Added: Mon Feb 02 2009, Hits: 5481, Reviews: 0 Write review Cached -
Isolation of Mouse Peritoneal Cavity Cells
(JoVE)
This video protocol describes a procedure for obtaining viable immune cells from the peritoneal cavity of mice, which then can be used for phenotypic analysis by flow cytometry and for different biochemical and immunological assays.
http://www.jove.com/details.php?id=1488
Added: Mon May 16 2011, Hits: 546, Reviews: 0 Write review -
Liver lymphocyte harvesting
(SurgeryResearch.com)
Harvesting lymphocytes from Murine Liver
http://www.surgeryresearch.com/Protocols/UM/Liver%...
Added: Wed Sep 25 2002, Hits: 1622, Reviews: 0 Write review -
Osteoblast Harvest from Mice
(SurgeryResearch.com)
Extract osteoblast cell from mice.
http://www.surgeryresearch.com/Protocols/OsteoMice...
Added: Wed Sep 25 2002, Hits: 341, Reviews: 0 Write review Cached -
Peripheral lymphocyte harvesting
(SurgeryResearch.com)
Harvesting lymphocytes from peripheral blood.
http://www.surgeryresearch.com/Protocols/UM/Periph...
Added: Wed Sep 25 2002, Hits: 497, Reviews: 0 Write review Cached -
Platelet Preparation
(Viral Immunity & Pathogenesis Group, the Université Catholique de Louvain)
This protocol is based on differential centrifugation that allows to separate platelets from blood cells.
http://www.icp.ucl.ac.be/mexp/vipg_plpre.html
Added: Sun Mar 21 2004, Hits: 3353, Reviews: 0 Write review Cached -
Preparation of Low Density, Collagenase-Digested Splenocytes
(Hedrick Lab, UCSD Cancer Center)
http://hedricklab.ucsd.edu/Protocol/SpleenPrep.htm...
Added: Tue Dec 10 2002, Hits: 1068, Reviews: 0 Write review Cached -
Purification of Progenitors from Skeletal Muscle
(JoVE)
This video protocol demonstrates the purification of skeletal muscle progenitors by flow cytometry from enzymatically dissociated muscle
http://www.jove.com/details.php?id=2476
Added: Mon May 16 2011, Hits: 568, Reviews: 0 Write review