Top : Cell Biology : Adhesion & Motility : Wound Healing (Cell Scratch) Assay

Summary: Wound healing or cell scratch assay is simple methods to study cell migration in vitro. This method mimics cell migration during wound healing in vivo. The basic steps involve creating a "wound" in a cell monolayer by scratching the surface of the culture vessel using a pipette tip, capturing the images at the beginning and at regular intervals during cell migration to close the wound, and comparing the images to quantify the migration rate of the cells. It is usually used to study the effects of cell-matrix and cell-cell interactions on cell migration.