Summary: Wound healing or cell scratch assay is simple methods to study cell migration in vitro. This method mimics cell migration during wound healing in vivo. The basic steps involve creating a "wound" in a cell monolayer by scratching the surface of the culture vessel using a pipette tip, capturing the images at the beginning and at regular intervals during cell migration to close the wound, and comparing the images to quantify the migration rate of the cells. It is usually used to study the effects of cell-matrix and cell-cell interactions on cell migration.
Protocols
- Wound healing assay (Light microscope facilities, University of Leicester)
Wound healing assay allows the researcher to study cell migration and cell interactions.
http://www.le.ac.uk/biochem/microscopy/wound-heali...
Added: Fri Feb 06 2009, Hits: 6046, Reviews: 0 Write review Cached - Wound Healing Assay Protocol
(Wallert and Provost Lab, Minnesota State University Moorhead)
Wound healing assays are used to estimate the migration and proliferation rates of different cells and culture conditions. These assays generally involve first growing a confluent cell monolayer. A small area is then disrupted and a group of cell destroyed or displaced by scratching a line through the layer. The open gap is then inspected microscopically over time as the cells move in and fill the damaged area...
http://web.mnstate.edu/provost/woundAssayProtocol....
Added: Sat Feb 16 2013, Hits: 2489, Reviews: 0 Write review -
Cell Scratch Assay Protocol
(Liang et al. Nature Protocol)
Cell scratch assay is a method to measure cell migration in vitro. The basic steps involve creating a "scratch" in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the scratch, and comparing the images to quantify the migration rate of the cells. In this protocol, Steps 1–9 describe the basic method of the in vitro scratch assay for measuring migration of cell populations.
http://www.nature.com/nprot/journal/v2/n2/full/npr...
Added: Sat Feb 16 2013, Hits: 2108, Reviews: 0 Write review Cached -
Endothelial wound healing (cell migration) assay
(The Science Advisory Board)
This is a simple assay that can be used in any cell culture lab setup to test the effect of different compounds on endothelial cell migration.
http://www.scienceboard.net/resources/protocols.as...
Added: Thu Feb 19 2009, Hits: 3776, Reviews: 0 Write review Cached