Top : Biochemistry : Amino Acids & Protein
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  • Basic Protein Chemistry Techniques (Salmon Lab, University of North Carolina at Chapel Hill, Department, of Biology)
    Basic techniques for protein chemistry including Coomassie Blue Stain and destain, dialysis tube preparation and more.
    http://www.bio.unc.edu/faculty/salmon/lab/protocol...
    Added: Tue May 14 2002, Hits: 5930, Reviews: 0  Write review   Cached
  • Acetylation (or Succinylation) of Amino Groups on Proteins (Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
    Derivitization of amino groups to remove the positive charge characteristics of the protein, for example to determine the importance of amino groups to a protein's function.
    http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
    Added: Sat Aug 31 2002, Hits: 1329, Reviews: 0  Write review   Cached
  • Amicon Stirred Ultrafiltration Cells (Sosnick Lab, University of Chicago)
    For protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW) cut-off are retained in cell, while water and solutes below the cut-off pass into the filtrate and out of cell.
    http://sosnick.uchicago.edu/amicon.html
    Added: Tue Aug 13 2002, Hits: 276, Reviews: 0  Write review   Cached
  • Angiotensin Protein Kinase Assay (Bart's Cookbook, The Salk Institute )
    This procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts.
    http://pingu.salk.edu/~sefton/Hyper_protocols/angi...
    Added: Mon Jul 29 2002, Hits: 768, Reviews: 0  Write review   Cached
  • Biosynthetic labeling (Sefton Lab, Salk Institute)
    http://pingu.salk.edu/~sefton/Hyper_protocols/bios...
    Added: Tue Aug 13 2002, Hits: 848, Reviews: 0  Write review   Cached
  • Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase (Hikmet Geckil's Lab)
    This is a fast, efficient and reproducible recovery procedure for periplasmic L-asparaginase from distinctly related gram-negative bacteria, Escherichia coli, Enterobacter aerogenes and Pseudomonas aeruginosa. As the method uses inexpensive organic solvent hexane and an aqueous salt solution, it is also highly cost-effective in comparison with the currently available techniques used for the release of this enzyme. As hexane is a highly water immiscible organic solvent, it can be removed easily from the top of the aqueous phase by a simple evaporation. Enzyme recoveries up to 3-fold with respect to sonication can be achieved with this system.
    Added: Wed Oct 22 2003, Reviews: 0  Write review  
  • Phosphoamino acid analysis (Sefton Lab, Salk Institute)
    Mark Kamps's method
    http://pingu.salk.edu/~sefton/Hyper_protocols/Paa....
    Added: Tue Aug 13 2002, Hits: 941, Reviews: 0  Write review   Cached
  • Preparing a Selenomethionyl Protein (Springer Lab, Harvard Medical School)
    The protocol describes how to prepare selenomethionyl (Se-Met) protein using a regular E.coli strain. Selenium can be used for phase determination in multi-wavelength anomalous diffraction (MAD) method. Se-Met can often replace methionine residues in a protein without affecting the protein's properties, therefore producing a protein advantageous for crystal structure solving. Also, the X-ray absorption edge of selenium is easily accessible by synchrotron radiation, making a Se-Met crystal ideal for collecting anomalous X-ray diffraction data.
    http://www.cbrinstitute.org/labs/springer/protocol...
    Added: Tue Aug 13 2002, Hits: 576, Reviews: 0  Write review   Cached
  • Protein arginine methylation PDF file (Methods.info)
    Step by step protocol with introduction to the background information about the method.
    http://www.methods.info/Methods/Protein_modificati...
    Added: Wed Sep 04 2002, Hits: 1175, Reviews: 0  Write review  
  • Purification of acidic phosphatase from mustard seedlings (Dr. Norbert H. Haunerland, Simon Fraser University)
    Phosphate esters are widely distributed in any organism. Nucleic acids, metabolic intermediates like glucose-6-phosphate, energy-rich substrates (AMP, creatine phosphate) are some obvious examples. While many metabolic intermediates are activated through the transfer of phosphate groups (e.g., by kinases) it is equally important that phosphate esters can also be rapidly broken down. The hydrolytic removal of phosphate groups from phosphoesters is catalyzed by phosphatases. This method describes isolation and purification of phosphatase from mustard seedlings
    http://www.sfu.ca/bisc/bisc-429/phosphatase.html
    Added: Sun Sep 22 2002, Hits: 809, Reviews: 0  Write review   Cached
  • Radioiodination of protein (Springer Lab, Harvard University)
    Radioiodination of proteins in solution and radioiodination of cell surface proteins
    http://www.cbrinstitute.org/labs/springer/protocol...
    Added: Tue Aug 13 2002, Hits: 641, Reviews: 0  Write review   Cached