Subcategories
Protocols
-
Acetylation (or Succinylation) of Amino Groups on Proteins
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
Derivitization of amino groups to remove the positive charge characteristics of the protein, for example to determine the importance of amino groups to a protein's function.
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 2868, Reviews: 0 Write review Cached -
Actin Preparation from Muscle
(Cooper Lab, Department of Cell Biology & Physiology Washington University in St Louis)
Preparation of conventional actin from skeletal muscle
http://www.cooperlab.wustl.edu/Methods/Actin_Prep_...
Added: Mon Aug 14 2006, Hits: 575, Reviews: 0 Write review -
Actin Purification from Nonmuscle Sources
(Cooper Lab, Department of Cell Biology & Physiology Washington University in St Louis)
http://www.cooperlab.wustl.edu/Methods/ActinPrepFr...
Added: Mon Aug 14 2006, Hits: 434, Reviews: 0 Write review -
Amicon Stirred Ultrafiltration Cells
(Sosnick Lab, University of Chicago)
For protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW) cut-off are retained in cell, while water and solutes below the cut-off pass into the filtrate and out of cell.
http://sosnick.uchicago.edu/amicon.html
Added: Tue Aug 13 2002, Hits: 1130, Reviews: 0 Write review Cached -
Angiotensin Protein Kinase Assay
(Bart's Cookbook, The Salk Institute )
This procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts.
http://pingu.salk.edu/~sefton/Hyper_protocols/angi...
Added: Mon Jul 29 2002, Hits: 1073, Reviews: 0 Write review Cached -
Basic Protein Chemistry Techniques
(Salmon Lab, Department of Biology, University of North Carolina at Chapel Hill)
Basic techniques for protein chemistry including Coomassie Blue Stain and destain, dialysis tube preparation and more.
http://www.bio.unc.edu/faculty/salmon/lab/protocol...
Added: Tue May 14 2002, Hits: 12438, Reviews: 0 Write review Cached -
Biosynthetic labeling
(Sefton Lab, Salk Institute)
http://pingu.salk.edu/~sefton/Hyper_protocols/bios...
Added: Tue Aug 13 2002, Hits: 1572, Reviews: 0 Write review Cached - Permeabilization of gram-negative bacteria with KPi/hexane for the release of L-asparaginase (Hikmet Geckil's Lab)
This is a fast, efficient and reproducible recovery procedure for periplasmic L-asparaginase from distinctly related gram-negative bacteria, Escherichia coli, Enterobacter aerogenes and Pseudomonas aeruginosa. As the method uses inexpensive organic solvent hexane and an aqueous salt solution, it is also highly cost-effective in comparison with the currently available techniques used for the release of this enzyme. As hexane is a highly water immiscible organic solvent, it can be removed easily from the top of the aqueous phase by a simple evaporation. Enzyme recoveries up to 3-fold with respect to sonication can be achieved with this system.
Added: Wed Oct 22 2003, Reviews: 0 Write review -
Phosphoamino acid analysis
(Sefton Lab, Salk Institute)
Mark Kamps's method
http://pingu.salk.edu/~sefton/Hyper_protocols/Paa....
Added: Tue Aug 13 2002, Hits: 1621, Reviews: 0 Write review Cached -
Preparing a Selenomethionyl Protein
(Springer Lab, Harvard Medical School)
The protocol describes how to prepare selenomethionyl (Se-Met) protein using a regular E.coli strain. Selenium can be used for phase determination in multi-wavelength anomalous diffraction (MAD) method. Se-Met can often replace methionine residues in a protein without affecting the protein's properties, therefore producing a protein advantageous for crystal structure solving. Also, the X-ray absorption edge of selenium is easily accessible by synchrotron radiation, making a Se-Met crystal ideal for collecting anomalous X-ray diffraction data.
http://www.cbrinstitute.org/investigators/springer...
Added: Tue Aug 13 2002, Hits: 519, Reviews: 0 Write review Cached