Anyone has experience with simple AFLP on plant? - (Oct/03/2005 )
Hi! I write for the fourth times because any one did not help me to resolve my problem: to succeed my aflp on a vegetable genetic materiel (sorghum)! Please, there is someone that can say me the causes that are able to be behind the failure of an aflp (in digestion, ligation, preamplification, selectamplification, migration. ..), or well to propose me a simple protocol on the plants in general or cereals? [font=Comic Sans Ms][size=5]
AFLP could fail at any of those steps. You can run a gel after each step except ligation to tell you if the step worked. You don't mention how your AFLP failed - did you get any bands?
I have used the AFLP protocol developed by the Michigan State University wheat group. You can get it on the web at www.msu.edu/user/hazensam/aflp/AFLPprotocolMSU.html It may well work on sorghum. Also, check the literature! Lots of AFLP mapping has been done in sorghum.