Blu native PAGE - (May/11/2001 )
Hallo everybody! I'm working on a protein that likely forms tetramers and dimers in equilibrium. The monomer molecular mass is around 12. I would like to move the equilibrium towards the dimers or tetramer but I have many difficult by gel filtration to obtain the separation of the two species: tipically on a Superose12 the sample elute in the trimer position instead of having two defined peaks. I've also tried to use a S-100 resin but results are not always clear. Now I'm trying a longer and sharper coloumn, but I heard about blue native PAGE as a system to resolve different species in solution. Anyone has experience in that and could give some suggestion? I don't know anything about this tecnique. Thank you very much Valeria
Hermann Schägger has developed the Blue Native system, look for his papers!