Can Taq pol elongate over biotin? - (Aug/31/2005 )
I'm wondering if Taq polymerase can elongate over an integrated biotin.
I know it is possible to incorporate biotin labelled nucleotides in PCR, but does the Taq actually work on templates that already have a biotin integrated?
Thanks for any help.
assuming biotin doesn't interfer with base pair formation, i would tell it's feasible.