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e. coli transformation efficiency... - (Aug/30/2005 )


I'm looking for informations concerning E. coli electrotransformation enhancment : DNA desalting? Ligase phenol extraction? DNA/cells incubation? ....


Not having too much salt is a good idea for electroporation. Not having too much protein around is also a good idea.

Is your reaction so inefficiƫnt that you need to enhance your electroporation performance? Most people I know just do reactions and then heat-shock transform the bacteria and get good results.