hope advices on improving the efficiency of library constrction! - ask for help on library constrction! (Aug/05/2005 )

hi, everyone:

I am using the M13 KE to construct a phage display library, I'm sure the ligation works well and, I even recovered the desired fragment (using the phage with the same size of insert as a control) and electroporated them to E.coli, but the library size is not very big, only 10 6-7. After that,, I have transformed the plasmid (containing my insert) to E.coli, and found that the transform efficiency is only a little higher than my ligation mixture. But now, for me, I hope the library size reach 1*10 9, and my next result will be fully determined by the library size.
Thus my question is:
How to improve the library size according to my detail?

All suggestion will be well appreciated!


Best wishes!