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priciples behind nucleic acid isolation - need this for additional lecture information (Aug/02/2005 )

I really need some information on the actual principles behind the following procedures to isolate nucleic acids: alkaline lysis , phenol chloroform extrcation , cesium chloride and silica dioxide.

What actually happens? why it happens ...the chemistry details ...........



I am not going to answer all your questions, but the basis of CsCl centrifugation is differences in density between, RNA, DNA and protein. CsCl is used because it forms a spontaneous gradient when centrifuged of the right density to separate the above three fractions.


DNA sequencing software

-Daniel Tillett-

try to ask specfic questions.... IT would be easier to answer to a particular point that doing all the chemistry of alkaline lyisi for ex... blink.gif


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