Question about activity of restriction enzymes - (Jul/28/2005 )
Does restriction enzymes have 5' to 3' activity, or can they read 'the other way', i.e. 3' to 5'?
don't know if my answer is the true one, but i think it's a problem of affinity. not reading in 5' to 3' or from 3' to 5', but just catch the appropriate sequence and kill it. The lovely couple of RE and target sequence...
you're right its an affinity question.
If you're looking for background information around restriction enzymes take a look in the technical reference guide of New England Biolabs.
Thanks for the replies! =D