application of co-IP - (Jul/27/2005 )
Hi guys,
I have a very basic question about co-IP. Is it only usable for detecting interactions between two known proteins, I mean you know these two proteins might interact, but you need to do a co-IP to confirm it, or you can also use it to identify novel interactions between proteins, just like you do a pull-down, run a gel, cut a band, and then do a MaLDI-TOF?
Thank you in advance
I have a very basic question about co-IP. Is it only usable for detecting interactions between two known proteins, I mean you know these two proteins might interact, but you need to do a co-IP to confirm it, or you can also use it to identify novel interactions between proteins, just like you do a pull-down, run a gel, cut a band, and then do a MaLDI-TOF?
Thank you in advance
it is not applicable to analyze as such: cut a band and do a malti.
Why not? Can you give me a reason
thanks
I have a very basic question about co-IP. Is it only usable for detecting interactions between two known proteins, I mean you know these two proteins might interact, but you need to do a co-IP to confirm it, or you can also use it to identify novel interactions between proteins, just like you do a pull-down, run a gel, cut a band, and then do a MaLDI-TOF?
Thank you in advance
it is not applicable to analyze as such: cut a band and do a malti.
hi
you can have non specific interaction occuring, or a third partner present in host that can mediate interaction of your two proteins... You need to confirm by a maldi if you can perform the technique, or by others approaches. I think Far westerns or interaction evaluation with two purified partners may enforce the result...
Hi fred_33,
Thank you for your answer. Do you mean it's possible to find a bunch of proteins interaction, not just two?? Actually that's exactly what I want to know
Of course, we'll need some functional studies to confirm the interaction
hi
you gonna find some interactions that are true (means that protein X can have more than Y as partners) and you can find also aberrant interactions or interactions that does not occurs in a human cell (as double hybrid is in yeast).
But on counter parts you can find interaction in X and Y because of the yast protein Z. And in further studies you gonna be crazy because you lack Z...
That why a positive interaction should be confirmed.
Negative interactions are not visible... so nevermind 