Reverse Transcription Kits - trying to decide which one to use (Jul/25/2005 )
I am getting ready to start real time PCR in my lab, but I am having troubles figuring out which kits to use. I am isolating RNA from muscle tissue using Trizol, then using the Poly A tract kit to pull out the mRNA, but then I am trying decide between the Invitrogen Superscript III first strand or the Promega Improm-II kit to make my cDNA, using random hexomer primers. I am using syber green master mix for the real time processing. Thanks in advance
I use Qiagen's Omniscript kit on a regular basis and have never had a problem with it.