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Extraction of collagen and elastin from tissue - Need protocol or advice (Jul/06/2005 )

Hi,

Would anyone have any experience in extracting salt soluble collagen, acid soluble collagen and pepsin isolated collagen from tissue. Extraction of elastin aswell. Im working with tissue weights of about 2grams per sample. I've looked for ages and cant find a comprehensive protocol on how to perform this procedure.

Any advice would be much appreciated.

Cheers

-galwaylad-

Hi Galwayguy,

We have been using two kits both of which have good protocols for collagen extraction from tissue. The first is the Chondrex "Human Type 1 Collagen detection kit" www.chondrex.com, and the second is the Sircol Solluble Collagen Assay by Biocolor at www.biocolor.co.uk

Both should have downloadable protocols and Sircol's is particularly clear and helpful.

Hope this helps! cool.gif

QUOTE (galwaylad @ Jul 6 2005, 01:44 PM)
Hi,

Would anyone have any experience in extracting salt soluble collagen, acid soluble collagen and pepsin isolated collagen from tissue. Extraction of elastin aswell. Im working with tissue weights of about 2grams per sample. I've looked for ages and cant find a comprehensive protocol on how to perform this procedure.

Any advice would be much appreciated.

Cheers

-drfinlay-