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problems with membrane fraction - (Jun/29/2005 )

I'm trying to prepare lysis buffer for renal membrane extraction. This method
was published in ''Hypertension'' (December 1997). Unfortunately,I haven't got
any results using protocol published in this paper. Could anibody please mail me
the adjective protocol of membrane fraction preparation? Should I know any
details during preparation?
Thank you.


Can't help specifically, but did you look up the references that the paper your are referring to used.

*pBluescript who once when back 3 generations of referred publications to find a 1958 publication on microfilm which provided the methology to help me develop and enzymatic assay*


Look up any paper by Carol Vandenberg.....the best membrane preps you'll ever get following her protocols