Dual-promoter plasmids? - Trying to transcribe sense and antisense strands.. (Jun/27/2005 )
I would like to transcribe both the sense and antisense strands of a clone in some type of high-copy number plasmid. Basically, I need a plasmid that has two identical (and ideally inducible) promoters just upstream of the MCS on each strand. It should look something like this:
Does anyone know of, or have experience with, such a plasmid?
For what it's worth, plasmid L4440 from the Fire RNAi construct kit contains exactly what I was searching for.