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Dual-promoter plasmids? - Trying to transcribe sense and antisense strands.. (Jun/27/2005 )

Hello all,

I would like to transcribe both the sense and antisense strands of a clone in some type of high-copy number plasmid. Basically, I need a plasmid that has two identical (and ideally inducible) promoters just upstream of the MCS on each strand. It should look something like this:


Does anyone know of, or have experience with, such a plasmid?



For what it's worth, plasmid L4440 from the Fire RNAi construct kit contains exactly what I was searching for.