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Simple method for lymphocyte extraction - (Jun/24/2005 )

Has anyone got an easy / straightforward / quick method for lymphocyte purification from whole blood ?

-wdavis-

in this article there is a protocol of enzymatic extraction with collagenase. But i can't access the full article so i don't know if it's what you really need unsure.gif

-fred_33-

QUOTE (wdavis @ Jun 24 2005, 09:23 PM)
Has anyone got an easy / straightforward / quick method for lymphocyte purification from whole blood ?


Hello! We are making lymphocytes extraction from whole blood using Lymphoprep from Axis-Shield. It is cheaper than Ficoll and very simple, you could see it in the protocol that I have attached.

-Patri-