Transfection in vivo - (Jan/15/2001 )
I need to transfect a plasmid into mouse liver and see the expression. Which type of liposome I should use? Is there any commercial product available?
Thanks in advance!
Yes there is a commercial product available - TransIT In Vivo Delivery System distributed by Panvera Corp. It works well with great expression levels in the liver.
Thank you for your information.
I got the detailed information about the kit. However, there is two doubts. One is the volumn injected (2.2ml for 10ug DNA. Is is too much for a normal mouse, say 20-30g? The other one is the strain of the mouse. Does it work better in some strains than others. How about SCID?
Thank you and looking forward to further information.