Which Kit is better for CO-IP? - (Jun/17/2005 )
I am now doing and I want to buy a. So many companes have CO-IP kits, I am realy confused. Anyone here has done CO-IP? Would you please give me some information-which kit is better?Thanks in advance.
While I personally haven't used the Pierce Seize Kits, I have known that used them, and they liked them a lot.
I would like to add, that unlike DNA which is generally easier to work with, proteins are by themselves unique beasts if you will. So that some kits won't work for your protein set, while another may. In my own experience, no kit would have worked, because my protein had unique binding properties...Your coIP may be easy, but it may also prove to be very empirical.
You do not actually need a kit for CoIP. It is truly simple for most applications.
Choice of detergent is the major issue. 1% Triton X100or 0.5% NP-40 are most
commonly used but sometimes they are too strong for interactions. Then try
digitonin or chapson which are milder and may preserve interaction better.