CDNA clean up protocol - (Apr/20/2005 )
Dear friend,
I will be very grateful if some one share with me a protocol of cDNA clean up. I mean how to purify cDNA from free nucleotide and primer.
Thank you
yong
-yongyk-
QUOTE (yongyk @ Apr 20 2005, 08:05 AM)
Dear friend,
I will be very grateful if some one share with me a protocol of cDNA clean up. I mean how to purify cDNA from free nucleotide and primer.
Thank you
yong
I will be very grateful if some one share with me a protocol of cDNA clean up. I mean how to purify cDNA from free nucleotide and primer.
Thank you
yong
Extract the reaction mixture once with phenol/chloroform and purify the cDNA using a Qiaquick spin column (qiaquick PCR purification kit, qiagen)
-Soltanlooh-
QUOTE (yongyk @ Apr 20 2005, 08:05 AM)
Dear friend,
Extract the reaction mixture once with phenol/chloroform and purify the cDNA using a Qiaquick spin column (qiaquick PCR purification kit, qiagen)
Extract the reaction mixture once with phenol/chloroform and purify the cDNA using a Qiaquick spin column (qiaquick PCR purification kit, qiagen)
-Soltanlooh-