possible coding of stop codon? - (Apr/18/2005 )

Hi,

I am trying to pull out the full length sequence of a possible new gene from a segment of 800 bp cDNA. There are two stop codons in the middle of the sequence. Is it possible that the stop codon can actually code some kind of aa, not just terminate the translation?

Thank you!

Jxy

Hi

It could be that the gene codes for several proteins through splice variants, hence several stop codons. It is now thought that many, if not all, genes have splice variants that code for different proteins. The other option is that there is a reading frame shift that misses those codons out or alters them into a coding protein.

Bob

also, certain organisms read a stop codon as an actual amino acid codon and vice versa.