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Question aboutbouy primary lymphocyte transfection? - (Apr/13/2002 )

I have to transfect the DNA into the primary thymocytes, just
isolated from mouse thymus.
I don't want to use ritrovirus system because I want to keep the thymocytes in naive type, no cell division.
Please give me some advises about which transfection system
I could use in this situation.


think about RNA transfection!

-Hans Lee-