help with microbiology!!! - (Apr/05/2005 )
Please help.....
Trying phage display, when we normally work on protein purification. Just electroporated TG1 cells with vector and insert and plated out on agar overnight.
Our protocol asks us to know a cells/ml concentration, but we only know how many colonies grew on our neat and serial dilution plates. Does this relate somehow? Or is there a step missing form our protocol.
Thanks in advance... please help if you can. Sorry if this seems a silly question!!
Sarah
-sarahsj-
QUOTE (sarahsj @ Apr 5 2005, 02:19 AM)
Please help.....
Trying phage display, when we normally work on protein purification. Just electroporated TG1 cells with vector and insert and plated out on agar overnight.
Our protocol asks us to know a cells/ml concentration, but we only know how many colonies grew on our neat and serial dilution plates. Does this relate somehow? Or is there a step missing form our protocol.
Thanks in advance... please help if you can. Sorry if this seems a silly question!!
Sarah
Trying phage display, when we normally work on protein purification. Just electroporated TG1 cells with vector and insert and plated out on agar overnight.
Our protocol asks us to know a cells/ml concentration, but we only know how many colonies grew on our neat and serial dilution plates. Does this relate somehow? Or is there a step missing form our protocol.
Thanks in advance... please help if you can. Sorry if this seems a silly question!!
Sarah
Hi, one single live bateria will form a colony on agar plate.This is represented as C.F.U/ml (colony forming unit) i.e. total no of bacteria in one ml that are capable of forming colonies on agar.
Total no of bateria includes live as well as dead bacteria. Total no of bateria can be counted by coulter counter/FACS/using Petroff-Hauser chamber.
I think in your case it is CFU that is important.
Devender
-dks0172-