Frozen TE saturated Phenol - Te Sturated Phenol getting frozen at 4 degree C (Apr/01/2005 )
Hi, am facing this problrm after I added the buffer provided with Sigma- TE saturated Phenol. It is getting frozen at 4 deg C.!!! When I checked the pH it was around 4!! so I added some more Buffer(pH 10) and added appropriate amount of HydroxyQuinnoline. But still the phenol is getting frozen and the pH has not reached 7-8. Can I keep the phenol at RT for thawing( generally up to 60min)? Do someone have any suggestion. PLease suggest.
phenol also does this in my hands, I usually let it sit at RT for it to melt and then use accordingly. That's normal!