PCR-AFLP - (Mar/02/2005 )
I am planning to do PCR-AFLP. Do I need to precipitate ligated DNA to remove unbound adapters which may serve as primers in the subsequent PCR? thanks for suggestions.
I don't think you have to precipitate the reaction. I have done AFLPs for several years and it always worked without.
also, is there a need for inactivating DNA ligase in water bath after restiriction-ligation step? thanks.
I've never heat-inactivated either the enzymes from the restriction or the ligase. Though I remember trying this when I had problems with the whole AFLP procedure once. It works without, but it wouldn't do harm either, I guess.