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Why transformation at lower temp? - How does lower temp improves transformation efficiency? (Feb/28/2005 )

I am carrying out KCM transformation. The insert is 8 kb in size. i want to transform it in to DH5alpha/XL1Blue. Is growing the cells at 18 deg C better ? How exactly temp is related to good transformation?


I believe the reason for growing at lower temperatures (especially for long inserts) is to prevent recombinants.