Why transformation at lower temp? - How does lower temp improves transformation efficiency? (Feb/28/2005 )
I am carrying out KCM transformation. The insert is 8 kb in size. i want to transform it in to DH5alpha/XL1Blue. Is growing the cells at 18 deg C better ? How exactly temp is related to good transformation?
I believe the reason for growing at lower temperatures (especially for long inserts) is to prevent recombinants.