how to determine the number of plates required for screening - (Apr/13/2002 )
> I plan to screen a cDNA library of human testis by homologous
> The protein to identify is estimated at 5% of the total protein in human
> testis. The library has 10(8) clones/ml, and we could screen up to 10(4)
> clones in a 90mm plate. Could you tell me how to determine the number of
> plates required for screening?
> Fei Xie MD
10-20 plates sounds like a good starting point. THis thing is abundantly expressed is it also abundantly transcribed?