Cloning problem - Insert DNA self-ligation? - (Jan/30/2005 )

I think I met the self-ligation problems as well

how did you solve this problem?

Thanks a lot....

Good luck.

http://bio.takara.co.jp/BIO_EN/catalog_d.asp?C_ID=C0008

According to the catalog

I think the Hind III restriction enzyme has the noticed Star activity. So that's why we got so many bands...

What do you think??

Cheers.....