Reuse TAE buffer and agarose gel? - yes or no... (Jan/27/2005 )
Our lab stock of Etbr is 10mg/ml, we use 5ul in a 100ml gel... so I guess your etbr is just quite dilute. I don't remember what Sambrook recommends.
Yeah one can use TAE buffer more than ten times for visulalization purposes, as Nick ve mentioned I ve seen people melting the gel and reusing that too..
In our experiments sometimes we can't follow the manual or the other guides exactly word by word, they ve mentioned that to promote the sales of their products.
Julianne, I guess you got some answers from the members for your query "How to cut cost in a molecular biolog lab?" through this question too.
have a nice time
I also tried reusing agarose gel by melting used gel. It works fine although it is not necessary for me. A concern I have is if the gel has been stained with EB, when it's heated, you may breathe EB into your body.