Aminoallyl Labeling - Efficiency of labeling (Jan/20/2005 )
I was wondering if any one has tried aminoallyl labeling before and what your opinions are in terms of qulaity of the results, the incorporation of the cy3/cy5 dyes and whether or not there is any dye bias issues to deal with when analyzing the microarray data?
in my experience amino-allyl labelling works well, although Cy3 ALWAYS works better than Cy5.
there is definately a dye bias for Cy3 in preference to Cy5. but as long as you do reverse labelling controls, you should be fine.