DGGE and reamplification - (Dec/29/2004 )
Hi,
I am trying to reamplify PCR products extracted from DGGE gel slices but with no success. The extraction method I am using is incubating the gel slices containing the DNA in 50 microl. DDW overnight at 4C. I would very much appreciate any help you offer regarding this problem.
Thanks very much
-drjjj-
Hi
We use the same overnight incubation in ddw but the next day we vortex the tube and prior to PCR stir the slice around in the water with a tip this works for us alternatively you could try freeze thaw treatment
good luck
-taggart-