ligation and transformation problems! Urgent! - (Nov/30/2004 )
QUOTE (Janina @ Dec 7 2004, 12:49 AM)
And what did you change at least?
I re-double cut the vector in a relative short time 1.5h.
I change the ratio of insert to vector. I usually set up the ligation based on the mass ratio of 3:1. But this time I change the mass ratio to molar ratio. I remember someone tells me that too much insert will inhibit the ligation.
ng of insert=ng of vector*(kb of insert/kb of vector)*(3/1)